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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Fig. 5

Comparison of H. polymorpha RB11 pC10-FMD (P FMD -GFP) cultures in synthetic Syn-6-MES medium containing 10 g L−1 glycerol and 0.12 M MES buffer with different concentrations of magnesium. The percentages refer to 3 g L−1 MgSO4·7H2O which is contained in the original medium. The presented signals are averages of parallel cultures in one MTP. The corresponding standard deviations are represented by the surrounding shaded areas. The number of investigated parallel cultures is specified in brackets in the legend behind the percentages. a Oxygen transfer rate (OTR). b Scattered light intensity at 650 nm. c Dissolved oxygen tension (DOT) measured via infrared oxygen-sensitive nanoparticles. d Fluorescence intensity of GFP (λex = 510 nm; λem = 532 nm). Cultivation conditions: 0.1 g L−1 infrared oxygen-sensitive nanoparticles; 48 round deep-well MTP without optodes, VL = 800 µL, n = 1000 rpm, shaking diameter d0 = 3 mm, 30 °C

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Microbial Cell Factories

ISSN: 1475-2859

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