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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Fig. 4

Dependency of the OTRmax (black circles) and kLa (red squares) on the filling volume for E. coli BL21 (DE3) pRhotHi YFP cultures in 48 round deep-well MTPs at 1000 rpm. Each measurement point is the average of minimum four cultures (compare Fig. 3). The standard deviation is presented by the corresponding errors bars. For comparison, in black the OTRmax is calculated based on an empirical correlation form literature [23]. The black dashed lines represent an error range of ±15% for the OTRmax calculated according to the empirical correlation. Cultivation conditions: synthetic Wilms-MOPS medium with 20 g L−1 glucose and 0.2 M MOPS buffer; 0.1 g L−1 infrared oxygen-sensitive nanoparticles; 48 round deep-well MTP without optodes, shaking diameter d0 = 3 mm, 37 °C

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