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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate

Fig. 1

Cultivation of E. coli BL21 (DE3) pRhotHi YFP on synthetic Wilms-MOPS auto-induction medium containing 0.5 g L−1 glucose, 2 g L−1 lactose, 5 g L−1 glycerol and 0.2 M MOPS buffer with (w/) and without (w/o) 0.1 g L−1 dispersed infrared oxygen-sensitive nanoparticles. 20 parallel cultures with (red) and 22 parallel cultures without (green) 0.1 g L−1 oxygen-sensitive nanoparticles were performed in the same MTP. The presented signals are averages of parallel cultures. The corresponding standard deviations are represented by the surrounding shaded areas. a Online monitoring of the oxygen transfer rate (OTR). The dissolved oxygen tension (DOT) is only available for cultures where oxygen-sensitive nanoparticles were applied (blue). b Microbial growth monitored via scattered light at 650 nm. c Fluorescence intensity of YFP (λex = 480 nm; λem = 522 nm). Cultivation conditions: 48 round deep-well MTP without optodes, VL = 800 µL, n = 1000 rpm, shaking diameter d0 = 3 mm, 37 °C

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