Table 2 Calculated strength of mvaS and Hmg1 RBS from strains from re-modulation libraries
Strains | Sequence of mvaS RBS | Strengtha of mvaS RBS | Sequence of Hmg1 RBS | Strengtha of Hmg1 RBS | INCREASE of β-carotene yield- CAR001 |
|---|---|---|---|---|---|
Hmg1-RBSL-2 | AGGAGAGAGAGG | 408 | AGGAGAAACAAC | 3877 | 0.93 |
Hmg1-RBSL-3 | AGGAGAGAGAGG | 408 | AGGAGGGAAAAA | 9975 | 0.98 |
Hmg1-RBSL-6 | AGGAGAGAGAGG | 408 | AGGAGAACAGCT | 3238 | 1.00 |
Hmg1-RBSL-M3 | AGGAGGGTATCG | 2159 | AGGAGGATAAAG | 7965 | 1.09 |
Hmg1-RBSL-11 | AGGAGAGAGAGG | 408 | AGGAGGAAAAAC | 12,720 | 1.11 |
Hmg1-RBSL-M1 | AGGAGACAAAAG | 1099 | AGGAGACAAAAG | 2656 | 1.26 |
Hmg1-RBSL-15 | AGGAGAGAGAGG | 408 | AGGAGGAAAAGG | 4855 | 1.31 |
Hmg1-RBSL-M10 | AGGAGAGGACTG | 612 | AGGAGAACAGCT | 3238 | 1.52 |