Skip to main content

Table 1 Analysis of refolding efficiency of secreted enzyme in the culture supernatant, relative to purified, soluble cellular enzyme

From: Proteins adopt functionally active conformations after type III secretion

 

Secreted

Purified

 

Protein

\( k_{cat}^{app} \) (s−1)

K M (µM)

\( k_{cat}^{app} \) (s−1)

K M (µM)

f fold

Bla

38 ± 4

28 ± 11

248 ± 11

42 ± 6

0.15 ± 0.02

PhoA

22 ± 2

100 ± 40

26 ± 2

230 ± 80

0.85 ± 0.10

  1. Uncertainty is given as the standard error and is propagated for the f fold calculation. All experiments were performed three times in biological replicate. Parameters for secreted samples were calculated for samples generated in LB media with 5 g L−1 NaCl