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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Proteins adopt functionally active conformations after type III secretion

Fig. 3

NaCl concentration in media affects secreted protein titer and folding efficiency. Both proteins studied are in the fusion format SptP-POI-2xFLAG-6xHIS. For all plots, unless specified, the mean of three biological replicates is plotted, except where noted by the symbol * to indicate two biological replicates. Error bars represent one standard deviation, unless noted. a i Plot of raw activity of secreted Bla as a function of growth media with representative western blot of analyzed samples. ii Plot of f fold for secreted Bla as a function of growth media. iii Plot of K M for secreted Bla as a function of growth media. Error bars represent the standard error. b. i Plot of raw activity of secreted PhoA as a function of growth media with representative western blot of analyzed samples. ii Plot of f fold for secreted PhoA as a function of growth media. iii Plot of K M for secreted PhoA as a function of growth media. Error bars represent the standard error

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