Table 4 Comparison of different methods using CRISPR/Cas9 system
From: Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
System composition | Transformation times | Cost | Editing efficiency | Time cost | Plasmid curing time | Labor intensity | |
|---|---|---|---|---|---|---|---|
pRed_Cas9_recA system this study | One plasmid | 1 | Low | 13.8 ± 7.9%(41 bp homologous arms) 100%(more than300 bp homologous arms) | 3 days (including plasmid construction, genome editing and plasmid curing) | 1 day | Low |
System established by Li [12] | Two plasmid and linear donr DNA | 2 | Medium | Around 100% | ND (estimated 4 days) | ND | Medium |
System established by Jiang [11] | Two plasmid and/or linear donr DNA | 2 | Medium | Around 100% | 4 days(without plasmid construction) | ND | Medium |
System established by Jiang [10] | Two plasmid and/or linear donr DNA | 2 | Medium | 65 ± 14% | ND (estimated more than 4 days without plasmid construction) | ND | Medium |