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Table 3 Efficiency of the two round consecutive editing with one-plasmid method

From: Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

 

Apparent efficiency

Editing efficiency

First round at poxB

Not determined

13.8 ± 7.9%

Second round at lacZ

89.7 ± 0.9%

19.9 ± 4.1%