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Table 3 The sequences of constructed theophylline riboswitches

From: Engineering an inducible gene expression system for Bacillus subtilis from a strong constitutive promoter and a theophylline-activated synthetic riboswitch

Theophylline riboswitches

Sequencesa

riboE1

ATACGACTCACTATA GGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCTGCTAAAGGAGGTAACAACAAG

riboE1-15

ATACGACTCACTATA GGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACCCTGCTAAAGGAGGTAACAACAAGCTGCAG b

  1. aItalic constant sequences; bolditalic theophylline riboswitch
  2. bUnderline inserted PstI site