Table 1 Strains and plasmids used in this study
Plasmids | Properties and characteristics | Reference |
|---|---|---|
pBSG03 | PsrfA, gfp, bla, Km r, shuttle vector | [20] |
pBSG26 | PaprE-HpaII, promoter, BSAP(aminopeptidase), bla, Km r, template for aprE Promoter (PaprE) | [19] |
pUC57B | pUC19 derivative, PaprE fused with modified theophylline riboswitch E (riboE1, 9-bp spacer), bla | This study |
pP43-gfp | pBSG03 derivative, ΔPsrfA::P43(from Bacillus subtilis 168), gfp, bla, Km r | This study |
pBSG04 | pBSG03 derivative, ΔPsrfA::PaprE, gfp, bla, Km r | This study |
pBSG61 | pBSG03 derivative, ΔPsrfA::PaprE-riboE1 | This study |
pBSG11 | pBSG61 derivative, ΔPaprE::P43, riboE1, gfp, bla, Km r | This study |
pBSG10 | pBSG11 derivative, insertion of PstI endonuclease immediately downstream of the 9-bp spacer (15-bp spacer), riboE1-15, gfp, bla, Km r | This study |
p43E-gus | pBSG11 derivative, Δgfp::gus, riboE1, bla, Km r | This study |
Strains | ||
E. coli JM109 | Lab stock | |
B. subtilis 168 | trpC2 | Lab stock |
BSG11 | B. subtilis 168 derivative, harbouring pBSG11 | This study |
BSG10 | B. subtilis 168 derivative, harbouring pBSG10 | This study |
BSG61 | B. subtilis 168 derivative, harbouring pBSG61 | This study |
BSG04 | B. subtilis 168 derivative, harbouring pBSG04 | This study |
BSG43 | B. subtilis 168 derivative, harbouring pP43-gfp | This study |
BSGgus | B. subtilis 168 derivative, harbouring p43E-gus | This study |