Anaerobic poly-3-d-hydroxybutyrate production from xylose in recombinant Saccharomyces cerevisiae using a NADH-dependent acetoacetyl-CoA reductase

Table 3 Acetoacetyl-CoA reductase (AAR) specific activity

Strain	Acetoacetyl-CoA reductase	Cofactor	V_max (µmol/min mg total protein)
TMB4443	C. necator	NADH	ND
TMB4443	C. necator	NADPH	9.9 ± 3.9
TMB4445	A. vinosum	NADH	184.7 ± 8.6
TMB4445	A. vinosum	NADPH	38.6 ± 2.2

200 µM of acetoacetyl-CoA and crude extracts of S. cerevisiae strains from overnight cultures cultivated in YNB-glucose were used. Averages and standard deviations were obtained from duplicate experiments
ND not detected

ISSN: 1475-2859