Fig. 6
Growth curves, carotenoid production and the effect of glucose on carotenoid production. Data from the wild-type, 385cyc8 − and 385tup1 − strains are shown. a Strains were cultured at 22 °C in MMv with 2% glucose. Top growth curves. Bottom specific carotenoid production at the S1, S2, S3, S4 and S5 growth phases (described in the text). The contents of astaxanthin and of other carotenoids (including neurosporene, γ-carotene, β-carotene, echinenone, hydroxy-echinenone and phoenicoxanthin) in each sample are represented in black and white, respectively. b Top growth curves. Middle total carotenoids produced per volume of culture during 24 h after glucose treatment. Bottom specific carotenoid production [(µg of carotenoid) (g yeast dry weight)−1] relative to the values before treatment (T0). YDW yeast dry weight. In all cases, values correspond to the average of three independent cultures, and error bars represent the standard deviation. *p < 0.05 and **p < 0.01, Student’s t test. Significant differences between µ values, according to ANOVA and Tukey’s post-test (α = 0.05; p ≤ 0.05), are represented by different letters (a–c)