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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8–Tup1 involved in catabolic repression

Fig. 3

Sequence analysis of the X. dendrorhous Cyc8 and Tup1 predicted proteins. a Cyc8: (i) scheme of the deduced Cyc8 protein, in which ten TPR motifs (I–X) were identified; (ii) the alignment of TPR motif IV (residues 169 to 203 of the X. dendrorhous Cyc8 protein) is shown as an example. b Tup1: (i) scheme of the predicted Tup1 protein. Q1 and Q2 represent two glutamine-rich regions; (ii) sequence alignment of the identified TupN domain (residues 1 to 75 of the X. dendrorhous Tup1 protein); (iii) sequence alignment of the identified WD40 motif III (residues 430 to 470 of the X. dendrorhous Tup1 protein) is shown as an example. Alignments are colored by identity percentage as follows: black 100% identity, grey > 80% identity, light grey > 60% identity and white < 60% identity. Additionally, red arrows above some residues indicate residues required for the formation of the secondary structures of the TPR (a helix-turn-helix (HTH) structure) and WD40 (four antiparallel β sheets) motifs. For TPR alignment, the Cyc8 protein sequences of X. dendrorhous [KX517902], C. neoformans [XP_567714.1], C. gattii [KIR43866.1], U. maydis [XP_011391826.1], C. cinerea [XP_001828491.2], A. fumigatus [XP_755516.1], A. oryzae [XP_001821909.1], A. niger [XP_001399530.1], A. dermatitidis [EEQ 87432.1], T. rubrum [XP_003236691.1], U. reesii [XP_002583327.1], S. pombe [NP_596609.1], and S. cerevisiae [AJQ13886.1] were used. For TupN and WD40 alignments of the Tup1 protein, Tup1 sequences of X. dendrorhous [KX517903], L. bicolor [XP_001881168.1], T. asahii [EJT51849.1], C. neoformans [AFR95667.1], C. gattii [XP_003194397.1], U. reesii [XP_002543629.1], P. marneffei [AAL99251.1], A. dermatitidis [XP_002620234.1], A. nidulans [CBF70872.1], A. kawachii [GAA92251.1], A. niger [XP_001396553.2], A. fumigatus [XP_747562.1] and S. cerevisiae [EGA87747.1] were used. The GenBank or NCBI reference sequence is indicated in brackets

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