Fig. 3

Spectrum and stoichiometry of inhibition of purified Siropins. a Effect of Siropin1 and 2 on HNE human neutropil elastase, PR3 proteinase 3, PPE porcine pancreatic elastase, BPT bovine pancreatic trypsine, BPC bovine pancreatic chymotrypsine and Sub subtilisine. The activity of each protease was assayed alone (green), pre-incubated with Siropin 1 (blue) or with siropin 2 (red). Measured protease activities without serpins were defined as 100%. Error bars represent the standard deviation from three independent experiments. b and c Stoichiometry of inhibition of Siropins. Fractional protease activity of HNE (b) and PR3 (c) was plotted after incubation with various amounts of Siropin 1 (Dashed, ^_o^_) or Siropin 2 (Continuous, ^_∆^_)