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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Siropins, novel serine protease inhibitors from gut microbiota acting on human proteases involved in inflammatory bowel diseases

Fig. 2

Electrophoretic, size exclusion chromatography and mass spectrometry analysis of the purified Siropins. a and c SDS-PAGE of purified Siropin 1 and Siropin 2, respectively. b and d Western blot detection of purified Siropin 1 and Siropin 2, respectively. Lane M protein marker (molecular mass in kilodaltons); lane 1 and 2 purified Siropin 1; lane 3 and 4 purified Siropin 2. e Size exclusion chromatography analysis of the purified recombinant proteins shows a single peak of 50 kDa for Siropin 2 (RT 15 min, continue line) and 45 kDa for Siropin 1 (RT 15.5 min, dashed line) using protein markers of 669 kDa (TR 8.9 min), 440 kDa (TR 11.3 min), 158 kDa (TR 13.2 min), 75 kDa (TR 14.1 min), 44 kDa (TR 15.3 min) and 29 kDa (TR 16.9 min). f and g Mass spectrometry analysis of the Siropin 1 and Siropin 2, respectively

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Microbial Cell Factories

ISSN: 1475-2859

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