Table 1 Primer sequences for psgRNA plasmid construction
Primers | Sequence (5′–3′) |
|---|---|
Primers for sgRNA spacer to target eyfp | |
 Ec-Φ_F | GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-P1_F | ATTAATTGTCAATTCGAAACGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-NT1_F | CCGTCCAGCTCGACCAGGATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-NT2_F | GCGCTCCTGGACGTAGCCTTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec_R | ACTAGTATTATACCTAGGACTGAGCTAGC |
Primers for sgRNA spacer to target glgc, sdhA and sdhB | |
 Ec-glgc1_F | TTGGCGCGCTGTTTGGTTAGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-glgc2_F | AGAGGTTGTAGGTCTGACTGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-sdhA1_F | TAATCAACGGCAATGTGTCAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-sdhA2_F | GCCCTGAGCCGCCACGCTATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-sdhB1_F | AGATCGTGACTGCAGGAATAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec-sdhB2_F | GCCGCCAAATCTTAAATTTCGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
 Ec_R | ACTAGTATTATACCTAGGACTGAGCTAGC |