Fig. 5

Effects of CRISPRi-mediated suppression on glycogen accumulation and succinate production. a Glycogen titer (µg/OD₇₃₀). b Succinate titer (mg/l). For glycogen analysis, Km^R/Cm^R cells were cultured to OD₇₃₀ ≈ 0.7–1.3, centrifuged and cultured in shake flasks containing 40 ml nitrogen-deplete (0×N) BG-11 for 2 days and subjected to glycogen analysis. To analyze succinate titer, PCC 7942 cells cultured in shake flasks to stationary phase (OD₇₃ ≈ 2.0) were centrifuged, and resuspended in 20 ml nitrate-free BG-11 medium. After centrifugation, the cells were resuspended in 40 ml nitrate-deplete (0×N) BG-11 medium containing Km/Cm and cultured in shake flasks. The WT cells were cultured in the nitrate-deplete (0×N) BG-11 or BG-11 (nitrate-replete) (1×N) medium