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Fig. 4 | Microbial Cell Factories

Fig. 4

From: CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942

Fig. 4

Effective suppression of endogenous genes by CRISPRi. a glgc expression. b sdhA expression. c sdhB expression. PCC 7942 cells were transformed with pSdCas9 first for integration into NSI site, and were transformed again with individual psgRNA plasmids for integration into NSII site. The KmR/CmR colonies were transferred to 40 ml BG-11 medium containing Km/Cm and cultured to OD730 = 0.7–1.3, and 5 ml cells were sampled for qRT-PCR analysis. The expression levels were normalized to those in the WT cells

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