Table 1 Cell growth, glycerol consumption and acetate accumulation at different induction times
From: Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system
Content | Induction time (h) | Straina | |||
|---|---|---|---|---|---|
S15A-0 | S15A-1 | S15A-2 | S15A-4 | ||
OD b600 | 0 | 5.93 ± 0.68 | 6.45 ± 0.48 | 3.45 ± 0.04 | 5.22 ± 0.44 |
12 | 7.02 ± 0.15 | 6.63 ± 0.35 | 6.43 ± 0.10 | 6.53 ± 0.22 | |
24 | 6.61 ± 0.13 | 6.82 ± 0.18 | 6.23 ± 0.12 | 6.94 ± 0.33 | |
Glycerolb (g/L) | 0 | 12.89 ± 0.00 | 12.89 ± 0.00 | 9.78 ± 0.68 | 12.52 ± 0.46 |
12 | 12.62 ± 0.31 | 12.26 ± 0.90 | 12.22 ± 0.12 | 12.60 ± 0.41 | |
24 | 12.89 ± 0.00 | 12.89 ± 0.00 | 12.89 ± 0.00 | 12.89 ± 0.00 | |
Acetateb (g/L) | 0 | 2.65 ± 0.55 | 2.84 ± 0.22 | 3.81 ± 0.68 | 3.48 ± 0.52 |
12 | 3.28 ± 0.35 | 3.95 ± 0.50 | 3.36 ± 0.20 | 4.09 ± 0.21 | |
24 | 3.40 ± 0.18 | 3.66 ± 0.22 | 3.22 ± 0.64 | 3.34 ± 0.42 | |