Fig. 5From: Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas systemProduction of PHB with gltA regulated using endogenous CRISPRi. Strains were cultured in 50 mL M9 medium containing 3% (v/v) glycerol. The inducer l-arabinose was added at 0 or 24 h at a concentration of 0.2% (g/v). The error bars indicate the standard deviations of three independent measurementsBack to article page