Fig. 1From: Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas systemCRISPRi process used in this study. The crRNA was expressed on a plasmid and Cascade was activated for expression with the constitutive promoter J23119. Cascade mediates the maturation of crRNA and forms complex with the crRNA. The complex then binds to a target site to disturb transcription. RNAP indicates RNA polymerase, PAM indicates protospacer adjacent motifBack to article page