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Fig. 6 | Microbial Cell Factories

Fig. 6

From: The effect of protein acetylation on the formation and processing of inclusion bodies and endogenous protein aggregates in Escherichia coli cells

Fig. 6

The IbpA/B molecular chaperones are overproduced in ΔackA-pta cells. Bacteria were grown as described in the legend to Fig. 5. a Whole-cell extracts and purified aggregates were resolved by SDS-PAGE and submitted to immunodetection using antibodies against ClpB, DnaK, GroEL, DnaJ, and IbpA/B molecular chaperones. Both IbpA and IbpB migrated in the polyacrylamide gel as one band. Volumes corresponding to equal amounts of bacteria were loaded onto the gels. b Purified aggregates were visualized by Coomassie staining

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