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Fig. 5 | Microbial Cell Factories

Fig. 5

From: The effect of protein acetylation on the formation and processing of inclusion bodies and endogenous protein aggregates in Escherichia coli cells

Fig. 5

Aggregation of endogenous E. coli proteins in ΔcobB and ΔackA-pta strains. Bacteria were grown at 37 °C in LB medium supplemented with 0.4% glucose. After 32 h, the cells were collected, resuspended in fresh LB medium without glucose, and incubated for 1 h at 37 °C. a Protein aggregates were isolated as described in “Methods” section, resolved by SDS-PAGE, and visualized by Coomassie staining or immunodetected using anti-acetyl lysine antibodies (Abcam). Volumes corresponding to equal amounts of bacteria were loaded onto the gels. b The level of aggregated proteins in relation to total protein in cell extracts was calculated on the basis of densitometry. Error bars represent the standard deviation of three values

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