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Table 1 Growth and ATF-SAP production parameters in P. pastoris recombinant cells during fed-batch fermentation

From: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera

 

Growth rate (h−1)

Glycerol uptake rate (g/l/h)

Correlation OD660nm/cell dry weighta

ATF-SAP yield (mg/g)b

ATF-SAP WT

0.42 ± 0.01

0.52 ± 0.01

y = 0.1853x2 + 0.3104x

0.98 ± 0.03

ATF-SAP KQ

0.44 ± 0.02

0.54 ± 0.02

y = 0.1956x2 + 0.4885x

0.86 ± 0.04

  1. ay = OD660nm; x = cell dry weight, mg
  2. bcalculated on yeast dry weight at 48 h after induction