Fig. 8

Cytotoxic activity of ATF-SAP was assayed on a uPAR over-expressing cancer cell line. Flow cytometric analysis of the cell surface expression of uPAR in the human leukemia U937 cell line using an anti-uPAR antibody (a). The secondary antibody used alone acted as control. b U937 cells were exposed to increasing concentrations of ATF-SAP WT (blue), ATF-SAP KQ (red) or seed saporin (green) and cell viability was measured by MTT assay after 48 (left panel) or 72 h (right panel). Error bars represent standard deviations from the mean of triplicate samples