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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera

Fig. 7

Characterization of ATF-SAP produced in P. pastoris and purified by IMAC. Yeast supernatants containing ATF-SAP WT and ATF-SAP KQ (pre) were treated with ammonium sulphate for protein precipitation and dialyzed (post). Protein products were analyzed by western blotting with an anti-saporin antibody (a). ATF-SAPs purified by IMAC were detected by silver staining (b) and western blotting (c) using an anti-saporin antibody as probe

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Microbial Cell Factories

ISSN: 1475-2859

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