Fig. 6

Fed-batch production of ATF-SAPs. Pichia pastoris growth curve (a), glycerol (b) and methanol (c) concentration were monitored before and after the induction of ATF-SAP WT (blue line) or ATF-SAP KQ (red line) expression. The green arrows indicate the methanol fed-batch. Error bars represent standard deviations from the mean of triplicate samples. The chimeric fusions secreted in the supernatants at the indicated time points were analyzed by western blot with anti-saporin antibody (d). Bands were quantified and expressed as fold increase (e). The asterisk indicates a different fed-batch production