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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera

Fig. 4

Shake flask production of ATF-SAP WT and ATF-SAP KQ. Pichia pastoris growth curve (a), glycerol (b) and methanol (c) concentration were monitored before and after the induction of ATF-SAP WT (blue line) or ATF-SAP KQ (red line) expression. The light blue arrows indicate the points at which pulses of methanol were delivered. Error bars represent standard deviations from the mean of triplicate samples. d The chimeric fusions in the supernatants at the indicated time points following induction were analyzed by western blotting using anti-saporin antibody as probe

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Microbial Cell Factories

ISSN: 1475-2859

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