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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera

Fig. 2

Time course analysis of the expression of ATF-SAP fusions in Pichia pastoris. Best-expresser clones for HisATF-SAP (a), ATF-SAPHis (b) and ATF-SAP (c) were induced and protein production assessed at different time points by western blotting using anti-saporin antibody as a probe. Equal amounts of culture supernatants were loaded. Seed derived SAP was used as a control. The presence of the histidine tag in ATF-SAPs was confirmed by western blotting using an anti-histidine antibody (d, left panel) and anti-SAP antibody as control (d, right panel)

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Microbial Cell Factories

ISSN: 1475-2859

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