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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Optimization of construct design and fermentation strategy for the production of bioactive ATF-SAP, a saporin based anti-tumoral uPAR-targeted chimera

Fig. 1

Constructs for the expression of ATF-SAP in Pichia pastoris yeast. Schematic representation of SAP (1) or ATF-SAPs (2–6) chimeric fusions. Native ATF (light blue), or optimized for the expression in yeast (ATFopt, blue) were fused through a 3 alanine linker to optimized SAP WT (red) or mutant SAP KQ (pink). A hexahistidine tag was appended either at the C- (construct 3) or at N-terminus (construct 4)

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Microbial Cell Factories

ISSN: 1475-2859

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