Fig. 3

GC and GC–MS analysis of n-dodecane extracts from E. coli expressing the MrBBS gene. a Total ion chromatograms of an authentic (−)-α-bisabolol standard (100 mg/L) and the n-dodecane extracts from the E. coli expressing MrBBS (pTSN-MrBBS) or containing an empty vector (pTrc99A). b Mass spectra of (−)-α-bisabolol standard. c Mass spectra of (−)-α-bisabolol produced in E. coli cells harboring the plasmid pTSN-MrBBS. Each cell was cultivated in LB medium overlaid with 20% (v/v) n-dodecane at 30 °C for 48 h