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Fig. 1 | Microbial Cell Factories

Fig. 1

From: An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria

Fig. 1

A schematic illustration of the procedure for the permeabilization and enzyme assays. 1. Cells were grown in volumes ranging from 1 l in a large flask to 0.2 ml in a 96-well plate. 2. Cells were harvested by centrifugation. 3. The pellets were resuspended in the B-PER reagent, typically in the volume of 50, 0.5, or 0.1 ml depending on the starting culture volumes, and incubated for 10 min on ice with a gentile agitation. 4. The permeabilized cells were pelleted by centrifugation and the B-PER reagent was removed. 5. The permeabilized cell pellets are resuspended in the Tris–HCl buffer and used directly for determination of the enzyme activities and the Chl a content

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