Fig. 1From: An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteriaA schematic illustration of the procedure for the permeabilization and enzyme assays. 1. Cells were grown in volumes ranging from 1 l in a large flask to 0.2 ml in a 96-well plate. 2. Cells were harvested by centrifugation. 3. The pellets were resuspended in the B-PER reagent, typically in the volume of 50, 0.5, or 0.1 ml depending on the starting culture volumes, and incubated for 10 min on ice with a gentile agitation. 4. The permeabilized cells were pelleted by centrifugation and the B-PER reagent was removed. 5. The permeabilized cell pellets are resuspended in the Tris–HCl buffer and used directly for determination of the enzyme activities and the Chl a contentBack to article page