Fig. 1
Schematic representation of the linearized expression plasmids a pSK275paf for the transformation in P. chrysogenum and b the T-DNA region of pBHt2_PAF binary vector for the A. tumefaciens mediated transformation of P. digitatum. Colour code of the expression cassette: black, paf 5′ UTR (promoter); green, paf 3′ UTR (terminator); yellow, gene of interest to be expressed (pre-pro sequence included); c ClustalW (BioEdit) alignment of the pre-pro proteins PAF[Pc] (equivalent to PAF[Pd]), PAFF31N, PAFY48Q and NFAP[Pc]. Top: PAF-specific pre-pro sequence; Bottom: mature proteins. The amino acid positions are numbered. Distinct amino acids are represented in colour code, identical amino acids are aligned in coloured dots, cysteine residues are boxed. The arrow indicates the predicted signal sequence cleavage site, the first amino acid of the mature proteins is marked with an asterisk. GOI gene of interest, hph hygromycin resistance gene cassette, ptrA pyrithiamine resistance gene cassette, ampR ampicillin resistance gene cassette, LB/RB left/right borders, F1 ori single-stranded phagemid origin; ColE1 origin, plasmid origin of replication, NotI restriction site for linearization of pSK275paf