Skip to main content

Table 1 Screening of carbonyl reductases for 3,5-BTAP reduction

From: Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol

Enzyme

Sources of strains

Conversions (%)

ee (%)

CR-1

Synechocystis sp. PCC 6803

43.1

>99(R)

CR-2

Lactobacillu kefir DSM 20587

97.6

>99(R)

CR-3

Lactobacillu kefir DSM 20587

15.3

>99(S)

CR-4

Gordonia polyisoprenivorans CCTT 137

13.6

>99(S)

  1. Substrate (50 mM), resting cells (50 g/L), and isopropanol [5% (v/v)] as co-substrate were mixed in PBS (100 mM, pH 7.0). The reaction was performed with shaking at 220 rpm and 30 °C for 24 h
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us