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Table 2 PHBHHx production by phaJ gene expression-controlled strains

From: Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator

Strain

Dry cell weight (mg/mL)

PHA content (% dry cell weight)

PHA (mg/mL)

Real cell massa (mg/mL)

3HHx composition (mol%)

005dZ126

19.1 ± 0.1

84.2 ± 0.2

16.1 ± 0.1

3.0 ± 0.1

2.8 ± 0.1

CnC1A

19.3 ± 0.2

84.7 ± 2.0

16.4 ± 0.2

2.9 ± 0.4

5.3 ± 0.1

CnTRPDA

19.1 ± 0.6

84.2 ± 2.7

16.1 ± 0.0

3.0 ± 0.6

7.8 ± 0.0

CnTRPA

19.1 ± 0.3

82.3 ± 1.5

15.8 ± 0.0

3.4 ± 0.3

8.6 ± 0.1

CnUV5DA

19.2 ± 0.5

83.4 ± 0.9

16.0 ± 0.3

3.2 ± 0.3

9.1 ± 0.0

CnUV5A

18.7 ± 0.8

84.0 ± 1.1

15.7 ± 0.4

3.0 ± 0.3

9.9 ± 0.2

CnTRCDA

19.1 ± 0.5

82.1 ± 0.2

15.7 ± 0.3

3.4 ± 0.1

9.6 ± 0.0

CnC1DB

18.8 ± 0.2

85.0 ± 0.6

16.0 ± 0.1

2.8 ± 0.2

5.8 ± 0.2

CnC1B

19.3 ± 0.5

84.2 ± 0.7

16.3 ± 0.3

3.0 ± 0.2

6.7 ± 0.6

CnTRPB

18.8 ± 0.1

84.7 ± 1.3

15.9 ± 0.3

2.9 ± 0.2

10.0 ± 0.1

CnUV5B

19.0 ± 0.0

84.1 ± 1.9

16.0 ± 0.3

3.0 ± 0.4

10.6 ± 0.0

CnTRCB

18.2 ± 0.4

84.0 ± 3.3

15.3 ± 1.0

2.9 ± 0.5

10.7 ± 0.1

  1. The cells were cultured in mineral salt medium with 0.129 w/v% (NH4)2SO4 and 1.5 w/v% palm kernel oil for 72 h. Data represent mean ± SD from three experiments performed in triplicate
  2. aReal cell mass = dry cell minus polyhydroxyalkanoates (PHAs)