Table 1 Bacterial strains and gene expression plasmids
Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
C. necator | ||
H16 | Wild type | ATCC17699 |
H16/ds | H16 derivative; ΔphaC1 | 17 |
005dZ126 | H16 derivative; ΔphaC1::phaC Ac NSDG, ΔphaZ1, ΔphaZ2, ΔphaZ6 | 27 |
CnC1A | 005dZ126, PphaC1RBS-phaJ4a | This study |
CnTRPDA | 005dZ126, PtrpdRBS-phaJ4a | This study |
CnTRPA | 005dZ126, PtrpRBS-phaJ4a | This study |
CnUV5DA | 005dZ126, PlacUV5dRBS-phaJ4a | This study |
CnUV5A | 005dZ126, PlacUV5RBS-phaJ4a | This study |
CnTRCDA | 005dZ126, PtrcdRBS-phaJ4a | This study |
CnC1DB | 005dZ126, PphaC1dRBS-phaJ4b | This study |
CnC1B | 005dZ126, PphaC1RBS-phaJ4b | This study |
CnTRPB | 005dZ126, PtrpRBS-phaJ4b | This study |
CnUV5B | 005dZ126, PlacUV5RBS-phaJ4b | This study |
CnTRCB | 005dZ126, PtrcRBS-phaJ4b | This study |
E. coli | ||
JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F’ [traD36 proAB + lacI q lacZΔM15] | Takara |
S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | ATCC47055 |
Plasmids | ||
pCUP3 | Stable plasmid vector in C. necator, Kmr | 17 |
pCUP3-PphaC1RBS-phaC1 | PphaC1RBS-phaC1 expression cassette cloned into pCUP3 | This study |
pCUP3-PphaC1dRBS-phaC1 | PphaC1dRBS-phaC1 expression cassette cloned into pCUP3 | This study |
pCUP3-RBS-lacZ | RBS-lacZ cloned into pCUP3 | This study |
pCUP3-PphaC1RBS-lacZ | PphaC1RBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PlacUV5RBS-lacZ | PlacUV5RBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PtrpRBS-lacZ | PtrpRBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PtrcRBS-lacZ | PtrcRBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-dRBS-lacZ | dRBS-lacZ cloned into pCUP3 | This study |
pCUP3-PphaC1dRBS-lacZ | PphaC1dRBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PlacUV5dRBS-lacZ | PlacUV5dRBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PtrpdRBS-lacZ | PtrpdRBS-lacZ expression cassette cloned into pCUP3 | This study |
pCUP3-PtrcdRBS-lacZ | PtrcdRBS-lacZ expression cassette cloned into pCUP3 | This study |