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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Evaluation of gene expression cassettes and production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with a fine modulated monomer composition by using it in Cupriavidus necator

Fig. 3

Structure of gene expression cassettes with the E. coli promoters, PtrcRBS, PlacUV5RBS, and PtrpRBS. The −35 and −10 promoter regions are overlined and labeled. Asterisks indicate the transcriptional start sites in E. coli. The 19-bp sequences derived from the region upstream of phaC1 are underlined, and the potential ribosome binding sites (RBS) are double-underlined. In their RBS disruptants, PtrcdRBS, PlacUV5dRBS, and PtrpdRBS, the potential RBS sequence (AGAGAGA) was changed to TCTCTCT

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