From: Biosynthesis of three N-acetylaminosugar-conjugated flavonoids using engineered Escherichia coli
Plasmids or E. coli strain | Relevant properties or genetic marker | Source or references |
---|---|---|
Plasmids | ||
pACYCDuet | P15A ori, Cmr | Novagen |
pCDFDuet | CDF ori, Strr | Novagen |
pETDuet | f1 ori, Ampr | Novagen |
pGEX 5X-2 | pBR322 ori, Ampr | GE healthcare |
pA-DHR | pACYCDuet + Pdeg and Preq from B. cereus ATCC 14579 | This study |
pC-DHR | pCDFDuet + Pdeg and Preq from B. cereus ATCC 14579 | This study |
pE-DHR | pETDuet + Pdeg and Preq from B. cereus ATCC 14579 | This study |
pA-DHR-D2 | pACYCDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2 | This study |
pC-DHR-D2 | pCDFDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2 | This study |
pE-DHR-D2 | pETDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2 | This study |
pA-6-DH | pACYCDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 | This study |
pC-6-DH | pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 | This study |
pE-6-DH | pETDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 | This study |
pA-6-DH-UXNAcS | pACYCDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98 | This study |
pC-6-DH-UXNAcS | pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98 | This study |
pE-6-DH-UXNAcS | pETDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98 | This study |
pC-6-DH-AmUGT | pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UGT10 from A. majus | This study |
pG-D2 | pGEX5x-2 + AtUGT78D2 | [17] |
Strains | ||
BL21 (DE3) | F− ompT hsdS B (r −B m −B ) gal dcm lon (DE3) | Novagen |
Bpgm | BL21(DE3) Δpgm | [17] |
BgalU | BL21(DE3) ΔgalU | [17] |
QS-1 | BL21(DE3) harboring pC-DHR-D2 | This study |
QS-2 | Bpgm harboring pC-DHR-D2 | This study |
QS-3 | BgalU harboring pC-DHR-D2 | This study |
QS-4 | BgalU harboring pC-DHR-D2 and pE-DHR | This study |
LNAGA-1 | BL21(DE3) harboring pC-6DH-D2-AmUGT | This study |
LNAGA-2 | BgalU harboring pC-6DH-D2-AmUGT | This study |
LNAGA-3 | Bpgm harboring pC-6DH-D2-AmUGT | This study |
QNX-1 | BL21(DE3) harboring pA-6-DH-UXNAcS and pG-D2 | This study |
QNX-2 | BL21(DE3) harboring pC-6-DH-UXNAcS and pG-D2 | This study |
QNX-3 | Bpgm harboring pA-6-DH-UXNAcS and pG-D2 | This study |
QNX-4 | Bpgm harboring pC-6-DH-UXNAcS and pG-D2 | This study |
QNX-5 | BgalU harboring pA-6-DH-UXNAcS and pG-D2 | This study |
QNX-6 | BgalU harboring pC-6-DH-UXNAcS and pG-D2 | This study |