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Table 1 Plasmids and strains used in the present study

From: Biosynthesis of three N-acetylaminosugar-conjugated flavonoids using engineered Escherichia coli

Plasmids or E. coli strain

Relevant properties or genetic marker

Source or references

Plasmids

 pACYCDuet

P15A ori, Cmr

Novagen

 pCDFDuet

CDF ori, Strr

Novagen

 pETDuet

f1 ori, Ampr

Novagen

 pGEX 5X-2

pBR322 ori, Ampr

GE healthcare

 pA-DHR

pACYCDuet + Pdeg and Preq from B. cereus ATCC 14579

This study

 pC-DHR

pCDFDuet + Pdeg and Preq from B. cereus ATCC 14579

This study

 pE-DHR

pETDuet + Pdeg and Preq from B. cereus ATCC 14579

This study

 pA-DHR-D2

pACYCDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2

This study

 pC-DHR-D2

pCDFDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2

This study

 pE-DHR-D2

pETDuet + Pdeg and Preq from B. cereus ATCC 14579 + AtUGT78D2

This study

 pA-6-DH

pACYCDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98

This study

 pC-6-DH

pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98

This study

 pE-6-DH

pETDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98

This study

 pA-6-DH-UXNAcS

pACYCDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98

This study

 pC-6-DH-UXNAcS

pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98

This study

 pE-6-DH-UXNAcS

pETDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UDP-N-acetylxylosamine synthase (UXNAcS) from B. cereus NVH 391–98

This study

 pC-6-DH-AmUGT

pCDFDuet + UDP-N-acetylglucosamine 6-dehydrogenase (UDP-GlcNAc 6-DH) from B. cereus NVH 391–98 + UGT10 from A. majus

This study

 pG-D2

pGEX5x-2 + AtUGT78D2

[17]

Strains

 BL21 (DE3)

F ompT hsdS B (r B m B ) gal dcm lon (DE3)

Novagen

 Bpgm

BL21(DE3) Δpgm

[17]

 BgalU

BL21(DE3) ΔgalU

[17]

 QS-1

BL21(DE3) harboring pC-DHR-D2

This study

 QS-2

Bpgm harboring pC-DHR-D2

This study

 QS-3

BgalU harboring pC-DHR-D2

This study

 QS-4

BgalU harboring pC-DHR-D2 and pE-DHR

This study

 LNAGA-1

BL21(DE3) harboring pC-6DH-D2-AmUGT

This study

 LNAGA-2

BgalU harboring pC-6DH-D2-AmUGT

This study

 LNAGA-3

Bpgm harboring pC-6DH-D2-AmUGT

This study

 QNX-1

BL21(DE3) harboring pA-6-DH-UXNAcS and pG-D2

This study

 QNX-2

BL21(DE3) harboring pC-6-DH-UXNAcS and pG-D2

This study

 QNX-3

Bpgm harboring pA-6-DH-UXNAcS and pG-D2

This study

 QNX-4

Bpgm harboring pC-6-DH-UXNAcS and pG-D2

This study

 QNX-5

BgalU harboring pA-6-DH-UXNAcS and pG-D2

This study

 QNX-6

BgalU harboring pC-6-DH-UXNAcS and pG-D2

This study