Strain, plasmid or primer | Relevant characteristic or sequence | References |
---|---|---|
Strains | ||
 E. coli DH5α | Host for plasmid maintenance | Laboratory strain |
 P. putida KT2440 | Wild type strain; used for heterologous expression of rhlAB operon | [44] |
 P. aeruginosa PAO1 | Wild type strain; used for amplification of rhlAB operon | [45] |
Plasmids | ||
 pBK-miniTn7-gfp2 | Gmr; P rnB P1 gfpAGA Delivery vector for miniTn7-gfp2 | [40] |
 pRK600 | Cmr; oriColE1 RK2-mob + RK2-tra + Helper plasmid in mating | [46] |
 pUX-BF13 | Apr; mob + oriR6K Helper plasmid providing Tn7 transposition function in trans | [47] |
 pJBA27 | gfpmut3* | [48] |
 pME6031 | Tcr; derivate of pME6010; cloning vector maintained in Pseudomonas strains without selection pressure | [37] |
 pVW10 | pME6031::gfpmut3* | This study |
 pVW13 | pVW10 with promoterless rhlAB | This study |
 pVW14 | pVW10 with rhlAB and its native promoter | This study |
 pVW12-SPL1 to pVW12-SPL120 | SPL in front of rhlAB | This study |
Primers | ||
 Gfp_Fwd | 5′-AGATAGAATTCAGATTCAATTGTGAGCGG-3′ | |
 Gfp_rev | 5′-AGATACTGCAGTATCAACAGGAGTCCAAGC-3′ | |
 SPL_rhlAB | 5′-AGATAAGATCTACN10TTGACAN16TATAATN6CCCG-ATCGGCTACGCGTGAACA-3′ | |
 rhlAB_rev | 5′-AGATAGAATTCATCACAGCAGAATTGGCCC-3′ | |
 SPL_con_rhlAB | 5′-AGATAAGATCTATCGGCTACGCGTGAACA-3′ | |
 Native_rhlAB | 5′-AGATAAGATCTACCCTGCCAAAAGCCTGA-3′ |