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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Biofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain Pseudomonas putida KT2440

Fig. 1

Construction and utilization of a synthetic promoter library in P. putida. a Outline of the plasmid construction strategy. The genes gfp, rhlA and rhlB indicate the green fluorescence protein, rhamnosyltransferase chain A and rhamnosyltransferase chain B, respectively. SPL indicate synthetic promoter library. The X in pVW12-SPLX represents any of the 120 synthetic promoters constructed in this study (see “Methods” section). b Gfp fluorescence measurement in a series of control strains without SPL. The control strains are wild type strain (P. putida) and strains containing an empty vector (pVW10), plasmid containing rhlAB operon (pVW13) with no promoter and the rhlAB operon with the native promoter from PAO1 (pVW14). The asterisks represent a significant difference (P < 0.05). c The different promoter strengths in the constructed SPL determined as Gfp intensities. The controls strains depicted in b are shown in grey. The columns represent mean value and the error bars indicate standard deviation. The Gfp intensities have been replicated 1–3 times. An enlarged picture of c can be found in Additional file 1: Figure S1 in which the name of the constructed promoters is shown

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