Real-time monitoring of the sugar sensing in Saccharomyces cerevisiae indicates endogenous mechanisms for xylose signaling

Table 4 Population heterogeneities for the hexose transporter-based biosensors during xylose cultivations

Strain	Population	FI fold changes (25 g/L xylose)			FI fold changes (50 g/L xylose)
Strain	Population	3 h	6 h	9 h	3 h	6 h	9 h
TMB3712 (HXT1p)	Single	1.24 ± 0.17	1.10 ± 0.10	1.00 ± 0.32	1.26 ± 0.36	1.23 ± 0.26	1.13 ± 0.31
TMB3712 (HXT1p)	% of total population	100	100	100	100	100	100
TMB3713 (HXT2p)	High FI	2.69 ± 0.10	3.90 ± 0.28	5.16 ± 0.060	3.23 ± 0.21	3.70 ± 0.020	4.52 ± 0.082
	% of total population	36 ± 0.1	43 ± 6.1	51 ± 2.1	32 ± 4.1	42 ± 0.5	49 ± 2.1
	Low FI	0.66 ± 0.004	0.58 ± 0.031	0.58 ± 0.004	0.71 ± 0.018	0.60 ± 0.017	0.58 ± 0.030
	% of total	64 ± 0.1	57 ± 6.1	49 ± 2.1	68 ± 4.1	58 ± 0.5	51 ± 2.1
TMB3714 (HXT4p)	High FI	4.40 ± 0.10	6.39 ± 0.15	7.76 ± 0.10	5.29 ± 1.22	6.01 ± 0.26	8.14 ± 0.024
	% of total population	19 ± 3.3	37 ± 3.2	42 ± 2.7	13 ± 8.3	29 ± 1.8	35 ± 0.2
	Low FI	1.02 ± 0.004	1.04 ± 0.007	1.21 ± 0.012	1.11 ± 0.11	1.07 ± 0.047	1.15 ± 0.068
	% of total population	8.1 ± 3.3	63 ± 3.2	58 ± 2.7	87 ± 8.3	71 ± 1.8	65 ± 0.2

Peak fold changes and population distributions (percent of total cell population) during cultivation in 25 and 50 g/L xylose in non-normalized data (for the normalized data from this experiment, cf. Additional file 1: Table S5). TMB3712 (HXT1p) displayed an increase in FI after already 3 h, but the analyzed samples were consistently distributed in a single peak population. TMB3713 (HXT2p) and TMB3714 (HXT4p) were highly heterogeneous on the FL-1 (GFP) channel with two distinct peaks (one with low and one with high FI). The histograms of TMB3713-3714 are also displayed in Fig. 5. The experiments were performed in biological and technical duplicates

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