Fig. 2
From: A novel methanol-free Pichia pastoris system for recombinant protein expression
The Δgut1-HpGCY1 strain has the potential to be developed to a novel methanol-free expression system. Concentrations of carbon sources were shown in brackets by the mass/volume percentage. M methanol; G glycerol; D glucose; DHA dihydroxyacetone. a Colorimetrical assay showing Aox activity in methanol, glycerol and DHA cultured wild-type strains. b Growth curves of WT, Δgut1, Δgut1-ScGCY1 and Δgut1-HpGCY1 strains on different concentrations of glycerol. c Colorimetrical assay showing Aox activity of the Δgut1-HpGCY1 strain under different glycerol concentrations and growth hours. Aox activity was not detected in glucose cultured Δgut1-HpGCY1 strain. Higher Aox activity corresponds to deeper red color in the colorimetrical assay. d Q-PCR comparing the gene transcription levels in Δgut1 and Δgut1-HpGCY1 strains grown on YNG medium. Folds were calculated towards the glycerol cultured WT strain. e Western blot showing the Aox protein levels of Δgut1 and Δgut1-HpGCY1 strains grown on glycerol. Glycerol or methanol cultured WT strains served as negative and positive controls, respectively