Fig. 1
From: A novel methanol-free Pichia pastoris system for recombinant protein expression
The Δgut1 and Δdak strains have abnormal Aox activity or limited cell growth. a The outline of glycerol and methanol metabolism pathways in methylotrophic yeasts [3, 28, 30]. Red arrows indicate the phosphorylation pathway of glycerol usage while blue arrows indicate the oxidation pathway. b Column 1 colorimetrical assay showing Aox activities in WT, Δgut1 and Δdak strains under different carbon sources. Deep red color suggests high Aox activity. White color suggests no Aox activity. Columns 2–4 spotting assay showing cell growth rates under different carbon sources. From column 2–4, spotting amount/concentration is 5 μL with 0.1, 0.01 and 0.001 OD600, respectively. D glucose; G glycerol; M methanol