Table 3 Fold changes of enriched transposon mutants in Tn-seq and growth rate increases of corresponding gene deletion mutants
| Â | Genea | Fold changeb | Growth rate % (P value) | Function |
|---|---|---|---|---|
Butanol | rob c | 15.3 | 32.8 (2E−3) | Right oriC-binding transcriptional activator |
rraA c | 11.4 | 8.2 (4E−2) | Ribonuclease E (RNase E) inhibitor protein | |
srmB | 7.0 | 16.3 (9E−3) | ATP-dependent RNA helicase | |
yecR | 4.9 | NS | Lipoprotein, function unknown | |
ydaE | 4.1 | NS | Rac prophage; conserved protein | |
aroL | 2.5 | 18.0 (1E−2) | Shikimate kinase II | |
iscR | 2.4 | 20.4 (2E−2) | Isc operon transcriptional repressor | |
Butyro-lactone | marB c | 12.0 | 30.3 (2E−2) | Mar operon regulator |
flxA c | 5.9 | ND | Qin prophage; uncharacterized protein | |
cspC | 5.1 | 30.9 (1E−2) | Stress protein, member of the CspA-family | |
sdhD | 4.2 | −40.4 (3E−3) | Succinate dehydrogenase | |
brnQ | 2.8 | NS | Branched-chain amino acid transport system 2 carrier protein | |
alx | 1.9 | 32.9 (8E−3) | Putative membrane-bound redox modulator | |
Butane-diol | cspC c | 10.8 | 23.3 (3E−5) | Stress protein, member of the CspA-family |
ydaE c | 8.3 | ND | Rac prophage; conserved protein | |
ydhU | 6.4 | 23.5 (1E−5) | Putative cytochrome b subunit of YdhYVWXUT oxidoreductase | |
yebG | 6.3 | −26.1 (3E−5) | DNA damage-inducible protein regulated by LexA | |
iscR | 4.3 | NS | Isc operon transcriptional repressor | |
ymfL | 4.1 | NS | e14 prophage; putative DNA-binding transcriptional regulator | |
Furfural | ydaE c | 8.2 | −31.1 (1E−4) | Rac prophage; conserved protein |
ydaG c | 7.9 | ND | Rac prophage; uncharacterized protein | |
ydhU | 7.2 | −22.8 (4E−4) | Putative cytochrome b subunit of YdhYVWXUT oxidoreductase | |
Decanoic acid | dusB c | 18.3 | ND | tRNA-dihydrouridine synthase B |
greA c | 17.4 | ND | Transcript cleavage factor | |
Geraniol | acrR c | 303.4 | ND | Transcriptional repressor |
yfeH c | 91.1 | ND | Putative inorganic ion transporter | |
Acetate | acrR c | 6.5 | 7.5 (9E−3) | Transcriptional repressor |
azuC c | 5.1 | ND | Acid-inducible small membrane-associated protein | |
ydaE | 4.3 | 17.0 (5E−4) | Rac prophage; conserved protein | |
yebG | 3.2 | NS | DNA damage-inducible protein regulated by LexA | |
cspC | 2.8 | 36.8 (2E−2) | Stress protein, member of the CspA-family | |
ydhU | 2.3 | NS | Putative cytochrome b subunit of YdhYVWXUT oxidoreductase | |
Itaconic acid | fnrS c | 7.5 | ND | sRNA anaerobic regulator |
tfaX c | 7.2 | ND | DLP12 prophage; predicted protein | |
rpsT | 7.0 | −22.2 (6E−5) | 30S ribosomal subunit protein S20 | |
tam | 5.3 | −23.3 (3E−4) | Trans-aconitate methyltransferase | |
racC | 4.4 | NS | Rac prophage; uncharacterized protein | |
Levulinic acid | azuC c | 7.0 | ND | Acid-inducible small membrane-associated protein |
rpsT c | 6.6 | ND | 30S ribosomal subunit protein S20 | |
Succinic acid | marB c | 8.6 | 19.5 (8E−3) | Mar operon regulator |
insH1 c | 6.1 | ND | IS5 transposase and trans-activator | |
brnQ | 4.1 | 39.8 (7E−4) | Branched-chain amino acid transport system 2 carrier protein | |
cpxA | 1.6 | 37.0 (3E−3) | Sensory histidine kinase. Two-comp. regulatory system with CpxR | |
Serine | cpxA c | 32.3 | ND | Sensory histidine kinase. Two-comp. regulatory system with CpxR |
marB c | 10.3 | NS | Mar operon regulator | |
Threo-nine | ydiN c | 245.7 | 13.6 (3E−2) | MFS transporter superfamily protein |
brnQ c | 61.7 | 60.2 (4E−4) | Branched-chain amino acid transport system 2 carrier protein | |
dusB | 12.5 | 59.0 (1E−4) | tRNA-dihydrouridine synthase B |