Fig. 4

Expression and localization of BcsZ. a BcsZ is expressed throughout the growth phase of S. Typhimurium UMR1(WT) and MAE97. Expression of BcsZ was analyzed after growth on LB without salt agar plates from 10 to 72 h. b Analysis of BcsZ expression in the supernatant and cell pellet indicates BcsZ to be mainly cell associated. c BcsZ is not a surface associated protein. Treatment of the bacterial pellet with different concentrations of proteinase K does not alter the BcsZ signal at 41.7 kDa. Detection of cytoplasmic OmpR (27.3 kDa) and periplasmic DsbA (22.9 kDa) were cell integrity controls