Fig. 3
The amount of β-glucan was increased by RHO1 overexpression. Relative β-glucan content in S. cerevisiae. 5 ml of yeast cells were incubated in SD-U medium for 48 h, and then transferred into fresh SD-U medium for 24 h (30 °C, 230 rpm). 2.5 × 106 cells were harvested and quantity of β-glucan was determined as described in the “Methods” section