Fig. 1

Growth phenotype of S. cerevisiae Δalg3Δoch1 was improved by RHO1 overexpression. a S. cerevisiae mutant strains were prespread on SD-U (lacking uracil) solid medium at 30 °C for 48 h before single colonies were selected and streaked on SD-U plates for 24 h. b Growth curve of W303A-pY26 (solid diamonds), W303A-FKS2 (solid round), W303A-RHO1 (snowflake), Δalg3Δoch1-pY26 (solid squares), Δalg3Δoch1-FKS2 (solid triangle), and Δalg3Δoch1-RHO1 (open squares). Cells were incubated in 5 ml of SD-U liquid medium for 24 h, and then the same amount of cells were collected and transferred into fresh 50 ml of SD-U medium for 66 h (30 °C, 230 rpm). c Yeast cells were cultivated in 5 ml of SD-U liquid medium for 48 h (30 °C, 230 rpm) and kept horizontally for 10 min before taking photos. d Morphology of mutant cells by microscope observation. Yeast Cells were incubated in 5 ml of SD-U liquid medium for 1 day (30 °C, 230 rpm). Cell morphology was determined by microscopy as described in the “Methods” section