Table 2 Substrate specificity of 6h-Aadh2p synthesized in A. adeninivorans G1212/YIC102-6H-AADH2

From: Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway

Substrate	Relative enzyme activity [%]
NAD⁺-dependent oxidation
Methanol	0.1
Ethanol	32
1-Butanol	100
1-Pentanol	84
1-Hexanol	84
Isopropanol	7
2-Butanol	23
1,2-Butanediol	2
2,5-Hexanediol	4
1-Phenylethanol	0
2-Phenylethanol	44
1-Nonanol^a	33
2-Nonanol^a	4
NADH-dependent reduction
Acetaldehyde	36
Butyraldehyde	83
Pentanal	100
Hexanal	100
Acetone	0
2-Butanone	0
4-Hydroxy-3-butanone	0
5-Chlorpentanone	0
2,5-Hexanedione	0
Ethyl 4-chloro-acetate	0
Phenylacetaldehyde	83
Acetophenon	0
2-Nonanon^a	0

Substrates were use at a concentration of 10 mM
^aSubstrates were used in a concentration of 1 mM

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