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Table 2 Substrate specificity of 6h-Aadh2p synthesized in A. adeninivorans G1212/YIC102-6H-AADH2

From: Aadh2p: an Arxula adeninivorans alcohol dehydrogenase involved in the first step of the 1-butanol degradation pathway

Substrate Relative enzyme activity [%]
NAD+-dependent oxidation
 Methanol 0.1
 Ethanol 32
 1-Butanol 100
 1-Pentanol 84
 1-Hexanol 84
 Isopropanol 7
 2-Butanol 23
 1,2-Butanediol 2
 2,5-Hexanediol 4
 1-Phenylethanol 0
 2-Phenylethanol 44
 1-Nonanola 33
 2-Nonanola 4
NADH-dependent reduction
 Acetaldehyde 36
 Butyraldehyde 83
 Pentanal 100
 Hexanal 100
 Acetone 0
 2-Butanone 0
 4-Hydroxy-3-butanone 0
 5-Chlorpentanone 0
 2,5-Hexanedione 0
 Ethyl 4-chloro-acetate 0
 Phenylacetaldehyde 83
 Acetophenon 0
 2-Nonanona 0
  1. Substrates were use at a concentration of 10 mM
  2. aSubstrates were used in a concentration of 1 mM
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Microbial Cell Factories

ISSN: 1475-2859

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