Fig. 7

Schematic representation of the transposon insertion at the gumD locus in the HePS biosynthesis deficient mutant strain of K. baliensis NBRC 16680. a Transposon insertion locus at the gumD gene. The first 279 bp represent the upstream region of the gumD gene (1,471,189–1,471,468 bp). The transposon insertion caused a duplication in the insertion region [direct repeat sequence (DR)] comprising 40 bp of the upstream chromosomal region of the gumD gene, as well as a part of the gumD sequence itself (1,471,428–1,471,620 bp) (the DRs are highlighted by black brackets, respectively). The mobile element has a total length of 1313 bp, which includes an ORF coding for a putative transposase (66–377), an ORF coding for a putative integrase (374–1252) and flanking regions (1–66; 1253–1313). The nucleotide sequence of the mobile element (1313 bp) is identically present at three further genomic regions of the K. baliensis NBRC 16680 chromosome (77,364–78,676 bp, 1,430,134–1,428,821 bp, 2,279,289–2,280,601 bp). At positions 1186–1221 bp of the mobile element a putative stem loop structure (dyad symmetry: 29.8 kcal/mol) could be predicted, which contains the restriction site for HpaI (5′-GTTAAC-3′; further explanation in text). The depicted genomic area was amplified with primers G4F_Fw and P4.2_Rev. b In I the protein sequence of a putative transposase with a predicted HTH_Tnp1 helix turn helix (bit score: 41.95, E-value: 5.57e-07) is depicted; II shows the protein sequence of a putative integrase and its predicted functional domains: HTH-like helix-turn-helix domain (bit score: 57.18, E-value: 1.44e-11) and integrase core domain (bit score: 104.65, E-value: 1.42e-28)