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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Lambda Red recombinase-mediated integration of the high molecular weight DNA into the Escherichia coli chromosome

Fig. 1

The fliK locus as the target for chromosomal integration of DNA. a Figure shows integration efficiency of the kanamycin resistance cassette (approximately 2 kb) into the fliK locus in the E. coli strains K12 MG1655 (K12) and BL21-DE3 (BL21). Means and standard deviations from three experiments are shown. b The sequence of the fliK locus of the E. coli chromosome, which was used as the target site for the chromosomal integrations. The figure shows the whole sequence of the fliK open reading frame. Integration primer parts are highlighted bold and underlined. The fliK sequence which was deleted from the engineered strains as a result of DNA integration by lambda Red-mediated homologous recombination is highlighted grey

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Microbial Cell Factories

ISSN: 1475-2859

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